yhaR [2019-06-07 09:40:15]

similar to enoyl CoA hydratase

locus: BSU09880

pI: 6.36

mw: 27.47 kDa

protein length: 255 aa

gene length: 768 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,061,491 1,062,258

The protein

Protein family

• enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|3111ECB66CD3CFF0494AA3DF4D646102CF3B0FA8|FadB]:
• [protein|D5CCFDE9312909B7E5B98876794569357791C76B|YngF]:

Structure

• [PDB|3P5M] (from ''Mycobacterium avium'', 33% identity) [pubmed|25613812]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|25B45D6A90AD5152FDA71718B14767EA86ECA15B|yhaR]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-B498 (yhaR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1497 NBRP B. subtilis, Japan]
• BKE09880 ([gene|25B45D6A90AD5152FDA71718B14767EA86ECA15B|yhaR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAAGCACCTCCATGT, downstream forward: _UP4_TAACAGATCAGCCGGAAAGC
• BKK09880 ([gene|25B45D6A90AD5152FDA71718B14767EA86ECA15B|yhaR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAAAGCACCTCCATGT, downstream forward: _UP4_TAACAGATCAGCCGGAAAGC

References

• 12850135,27766092